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semidry electro-transfer system  (Bio-Rad)


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    Structured Review

    Bio-Rad semidry electro-transfer system
    Semidry Electro Transfer System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/semidry+electro-transfer+system/trans+blot+sd+semi+dry+transfer+cell/pm38659349-90-15-21
    Average 90 stars, based on 1 article reviews
    semidry electro-transfer system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Exhaled breath condensate contains extracellular vesicles (EVs) that carry miRNA cargos of lung tissue origin that can be selectively purified and analyzed.
    Article Snippet: Proteins were then transferred to 0.2 μm polyvinylidene fluoride (PVDF) membranes (Bio-Rad, #1704156) using a semidry electro-transfer system (TransBlot Turbo v1.02, Bio-Rad) for 30 min at 25 V. Proteins were visualized using the stain-free blot protocol provided on a Chemi-DocTMMP (Bio-Rad) system to evaluate protein transfer and membranes were blocked using EveryBlot blocking buffer (Bio-Rad, #12010020) for 30 min. Membranes were incubated at 4◦C O/N with TBS-T (1x TBS, pH 6.8, 0.1% Tween20) diluted anti-mouse primary antibodies targeted against Apolipoprotein B (Novus Biologicals, Cat#MAB4124), Albumin (Novus Biologicals, Cat#MAB1455), CD63 (Abcam, Cat#ab59479), or with anti-rabbit primary antibodies targeted against Apolipoprotein A1 (Novus Biologicals, Cat#MAB36641), CD81 (Abcam, #ab233692) and CD9 (Abcam, #ab263023).

    Blocking Assay:

    Article Title: Exhaled breath condensate contains extracellular vesicles (EVs) that carry miRNA cargos of lung tissue origin that can be selectively purified and analyzed.
    Article Snippet: Proteins were then transferred to 0.2 μm polyvinylidene fluoride (PVDF) membranes (Bio-Rad, #1704156) using a semidry electro-transfer system (TransBlot Turbo v1.02, Bio-Rad) for 30 min at 25 V. Proteins were visualized using the stain-free blot protocol provided on a Chemi-DocTMMP (Bio-Rad) system to evaluate protein transfer and membranes were blocked using EveryBlot blocking buffer (Bio-Rad, #12010020) for 30 min. Membranes were incubated at 4◦C O/N with TBS-T (1x TBS, pH 6.8, 0.1% Tween20) diluted anti-mouse primary antibodies targeted against Apolipoprotein B (Novus Biologicals, Cat#MAB4124), Albumin (Novus Biologicals, Cat#MAB1455), CD63 (Abcam, Cat#ab59479), or with anti-rabbit primary antibodies targeted against Apolipoprotein A1 (Novus Biologicals, Cat#MAB36641), CD81 (Abcam, #ab233692) and CD9 (Abcam, #ab263023).

    Incubation:

    Article Title: Exhaled breath condensate contains extracellular vesicles (EVs) that carry miRNA cargos of lung tissue origin that can be selectively purified and analyzed.
    Article Snippet: Proteins were then transferred to 0.2 μm polyvinylidene fluoride (PVDF) membranes (Bio-Rad, #1704156) using a semidry electro-transfer system (TransBlot Turbo v1.02, Bio-Rad) for 30 min at 25 V. Proteins were visualized using the stain-free blot protocol provided on a Chemi-DocTMMP (Bio-Rad) system to evaluate protein transfer and membranes were blocked using EveryBlot blocking buffer (Bio-Rad, #12010020) for 30 min. Membranes were incubated at 4◦C O/N with TBS-T (1x TBS, pH 6.8, 0.1% Tween20) diluted anti-mouse primary antibodies targeted against Apolipoprotein B (Novus Biologicals, Cat#MAB4124), Albumin (Novus Biologicals, Cat#MAB1455), CD63 (Abcam, Cat#ab59479), or with anti-rabbit primary antibodies targeted against Apolipoprotein A1 (Novus Biologicals, Cat#MAB36641), CD81 (Abcam, #ab233692) and CD9 (Abcam, #ab263023).



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